NDD and the Gut Microbiome
We have developed inclusive and adapted protocols to examine sleep, gastrointestinal issues, and behaviours of concern in children with NDDs.
Children and youth with NDDs often experience co-occurring behaviours of concern (BoC), gastrointestinal (GI) issues, and sleep problems. Despite clear associations between these areas, there is a growing need for studies that use biological samples, such as stool and breath samples, to better understand how GI and sleep problems may contribute to the presentation of BoC in this population. However, standard research methods are often inflexible, requiring participants to remain still, follow complex instructions, or visit unfamiliar environments. These barriers can make participation difficult for children with NDDs and BoC, resulting in their exclusion from important research.
To address this gap, we developed and validated an inclusive, adapted protocol for biological sample collection through a pilot study involving children and youth with NDDs and co-occurring BoC. Our approach includes tailored protocols for breath and stool collection that recognize and accommodate the unique needs and abilities of this population. Protocols were refined across three collection time points through feedback from participating families.
Key innovations of our protocol include the active involvement of individuals with lived experience, flexible scheduling, reduced travel demands for families, alternative collection methods that reflect developmental needs, and hands-on demonstrations of the collection procedures. The protocol is currently being prepared for publication, and a follow-up paper focused on the results of the collected samples will follow.
Collaborators:
Dr. Kendall Corbin PhD, Assistant Professor, Department of Horticulture, University of Kentucky
Dr. Heather Bean PhD, Associate Professor, School of Life Science, Arizona State University